Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Oral Presentations

doi: 10.1002/term.1931

Figure Lengend Snippet: Figure 1 SEM surface image (a, 5009) of PGS-PCL scaffold containing encapsulated Res; Young’s Modulus (b); FTIR spectra (c); The expres- sion of MMP9 and MMP2 on EAhy926 endothelial cells under hypoxia conditions after 3 days (d).

Article Snippet: Cancer cell lines were then pre-grown in hydrogels for 5–7 days and then re-seeded into starPEG-heparin hydrogels functionalised with RGD, SDF-1, bFGF and VEGF as spheroids with HUVECs and MSC and grown for 14 days as a tri-culture in Endothelial Cell Growth Medium (ECGM; Promocell).

Techniques:

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Oral Presentations

doi: 10.1002/term.1931

Figure Lengend Snippet: Figure 1 (A) Tube-in-stent valve in (B) open (systolic) and (C) closed (diastolic) configuration. Materials and methods: The tubular constructs were moulded with fibrin and human umbilical vein cells (60 9 106/mL). They were sewn into a stent (Admedes Schuessler GmbH, Pfonzheim, Germany) and conditioned for 3 weeks in bioreactors. Endothelialization with human umbilical vein cells was performed during the last week of stimulation. After harvesting, the valves underwent crimping for 20 minutes to sim- ulate the catheter based-delivery. The tissue was analyzed by immuno- histochemistry, hydroxyproline assay, scanning electron microscopy (SEM). The performance of the valve was determined in terms of effec- tive orifice area and transvalvular pressure drop under aortic condi- tions. Mechanical properties were quantified as burst strength. Results: After dynamic conditioning the valves (n = 3) were fully func- tional with unobstructed opening (systolic phase) and complete closure (diastolic phase). Tissue analysis showed a homogeneous cell distribu- tion throughout the valve0s thickness and deposition of collagen types I and III oriented along the longitudinal direction. Immunohistochemical staining against CD31 and SEM revealed a confluent endothelial cell layer on the surface of the valves. The crimping procedure did not affect the valvular functionality in terms of effective orifice area and

Article Snippet: Cancer cell lines were then pre-grown in hydrogels for 5–7 days and then re-seeded into starPEG-heparin hydrogels functionalised with RGD, SDF-1, bFGF and VEGF as spheroids with HUVECs and MSC and grown for 14 days as a tri-culture in Endothelial Cell Growth Medium (ECGM; Promocell).

Techniques: Construct, Immunohistochemistry, Hydroxyproline Assay, Electron Microscopy, Immunohistochemical staining, Staining

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Oral Presentations

doi: 10.1002/term.1931

Figure Lengend Snippet: Figure 1 Necessary steps to transform the iLA to a longterm ambulatory lung assist device. To improve the hemo- and biocomaptiblity of the gasexchange mate- rial Polymethlypentene (PMP), we designed a strategy to seed the fibers with human endothelial cells. Therefore a cell adhesion promot- ing biochemical surface with benzophenone modified heparin and suit- able chemical side chains to perform both, peptide-coupling and covalent conjugation to PMP- membranes was developed. In order to provide cell adhesion sites RGD-peptides were coupled. Human dermal endothelial cells (HDMEC) were seeded to the surface and analyzed with FDA/PI. Results: We succeeded in miniaturization of all hardware components as well as in a new lid and inflow design of the gasexchanger to improve blood distribution (Figure 2a). Figure 2b shows FDA stained vital endothelial cells after 48 h on the PMP fibers under static cell cul- ture conditions condition (37°C, 5% CO2).

Article Snippet: Cancer cell lines were then pre-grown in hydrogels for 5–7 days and then re-seeded into starPEG-heparin hydrogels functionalised with RGD, SDF-1, bFGF and VEGF as spheroids with HUVECs and MSC and grown for 14 days as a tri-culture in Endothelial Cell Growth Medium (ECGM; Promocell).

Techniques: Conjugation Assay, Staining

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Oral Presentations

doi: 10.1002/term.1931

Figure Lengend Snippet: Figure 1 Co-cultures and triple-cultures were stained for the endothelial marker CD31 followed by quantitative assessment of vascular struc- tures influenced by the addition of EPC.

Article Snippet: Cancer cell lines were then pre-grown in hydrogels for 5–7 days and then re-seeded into starPEG-heparin hydrogels functionalised with RGD, SDF-1, bFGF and VEGF as spheroids with HUVECs and MSC and grown for 14 days as a tri-culture in Endothelial Cell Growth Medium (ECGM; Promocell).

Techniques: Staining, Marker

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Oral Presentations

doi: 10.1002/term.1931

Figure Lengend Snippet: Figure 2 (A) Co-cultures and triple-cultures on PU-HA after van Kossa staining (B) samples were stained for the endothelial marker CD31 (green) and myeloid marker CD11b (red) in 3D. Discussion and conclusions: Our findings demonstrate that endothelial progenitor cells support mature endothelial cells in the vascularization process in co-culture with MSC whereas the impact on bone formation still needs to be further defined. Acknowledgments: The authors want to thank Katrin Lange, Gabriele Nessenius, and Barbara Pavic for the excellent technical support Disclosures: Authors have nothing to disclose. References Shi et al. Eur Cell and Mater. 2014 Jan 25; 27:64–80.

Article Snippet: Cancer cell lines were then pre-grown in hydrogels for 5–7 days and then re-seeded into starPEG-heparin hydrogels functionalised with RGD, SDF-1, bFGF and VEGF as spheroids with HUVECs and MSC and grown for 14 days as a tri-culture in Endothelial Cell Growth Medium (ECGM; Promocell).

Techniques: Staining, Marker, Co-Culture Assay

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Oral Presentations

doi: 10.1002/term.1931

Figure Lengend Snippet: Figure 1 Phenotypic characterization of cultured BM-derived macro- phages treated with MSC-conditioned medium. Discussion and conclusions: Our results proved the potential of uncom- mitted MSCs to functionallly mobilize host cells into an induced bone regenerative niche in vivo. In particular, we demonstrated a cross-talk between implanted MSCs and host cells, such as macrophages as well as bone marrow-derived endothelial and mesenchymal precursors,

Article Snippet: Cancer cell lines were then pre-grown in hydrogels for 5–7 days and then re-seeded into starPEG-heparin hydrogels functionalised with RGD, SDF-1, bFGF and VEGF as spheroids with HUVECs and MSC and grown for 14 days as a tri-culture in Endothelial Cell Growth Medium (ECGM; Promocell).

Techniques: Cell Culture, Derivative Assay, In Vivo

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Oral Presentations

doi: 10.1002/term.1931

Figure Lengend Snippet: Figure 1 (A) Young’s Moduli at 20% strain show increased tunable stiff- ness with the addition of increasing TG. (B) Cell viability is conserved in Fibrin-ECM gels at all stiffness with aECM and lower (but still ~80%) in nECM gels. (C) CPCs interact with ECM (fluorescently labeled green, top) and generate significant cellular networks after 3 wks in culture (bottom). (D) PCR demonstrates increased endothelial differentiation in aECM gels that increases with increasing stiffness. (E) Fibrin-ECM gels are able to be injected into hearts (top) and are retained (Evans blue dye labeled, bottom).

Article Snippet: Cancer cell lines were then pre-grown in hydrogels for 5–7 days and then re-seeded into starPEG-heparin hydrogels functionalised with RGD, SDF-1, bFGF and VEGF as spheroids with HUVECs and MSC and grown for 14 days as a tri-culture in Endothelial Cell Growth Medium (ECGM; Promocell).

Techniques: Labeling, Injection